Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIW----TEDASRMEAMSLDYFLSYPRLFWPKF---KELFQMKMSGSFEPNEGHLLLAELE----KQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACG---ARYDL-------------PHLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAVMH--FDTLYEKLDQADLLLVIGTSLEVAPAR-FVPEDASLIPGMKKVIINLEPTYCDSLFDMVIHQKIGEFARSLGMKK 4UUA Chain:B ((13-258)) LTAFREHFAKAKHIAIITGAGVSAESGVPTFRGPGGFWRKWQAQDLATPEAFSRD-----PSLVWEFYHYRREVMRSKM-----PNPAHLAIAECEARLGQQGRSVVIITQNIDELHHRAGSKHVYEIHGSLFKTRCMSCGEVKANHKSPICPALDGKGAPDPNTKEARIPVELLPRCERKSCNGLLRPHVVWFGETLDSDILTAVERELEKCDLCLVVGTSSIVYPAAMFAPQVASR--GVPVAEFNMECTPATQRF-------------------

General information: TITO was launched using: RESULT: Template: 4UUA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 991 -93781 -94.63 -434.17 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -94.63 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_4UUA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UUA-query.scw PDB file : Tito_Scwrl_4UUA.pdb: