Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPEGKIIKALSGFYYVLDESEDSDKVIQCRGRGIFRKNKITPLVGDYVVYQAENDKEGYLMEIKERTNELIRPPICNVDQAVLVFSAVQPSFSTA-LLDRFLVLVEANDIQPIICITKMDLIEDQDTEDTIQAYAEDY-RNIGY---DVYLTSSKDQDSLADIIPHFQ----DKTTVFAGQSGVGKSSLLNAISPELGLRTNEISEHLGRGKHTTRHVELIHTS-GGLVADTPGFSSLEFTDIEEEELGYTFPDIREKSSSCKFRGCLHLKEPKCAVKQAVEDGELKQYRYDHYVEFMTEIKDRKPRY 3H2Y Chain:A ((64-225)) -----------------------------------------------------------------------NGIGKSDALVVKIVDIFDFNGSWLPGLHRFV-----GNNKVLLVGNKADLIPKSVKHDKVKHWMRYSAKQLGLKPEDVFLISAAKGQGIAELADAIEYYRGGKDVYVVGCTNVGKSTFINRMIKEFSDETENV-ITTSHFP----DLIDIPLDEESSLYDTPGIINHH---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3H2Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 668 -82620 -123.68 -558.24 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -123.68 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_3H2Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H2Y-query.scw PDB file : Tito_Scwrl_3H2Y.pdb: