Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSTLRKDLIELFSQAGNEFISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTEVMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGVWMSLILRPDIPLQKTPQLTLLAAVAVVQGIEEAAGIQTDIKWPNDILI----NGKKTVGILTEMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEKIDRAGVIQHILLCFEKRYRDYMTHGFTPIKLL--WESYALGIGTNMRARTLNG-TFYGKALGIDDEGVLLLETNEGIKKIYSADIELG 2EJ9 Chain:A ((2-231)) ------------------------------------------------------------------------------------EIIHLSEIDSTNDYAKELAKE-GKRNFIVLADKQNNGKGRWGRVWYSDEG-GLYFSMVL--DSKLYNPKVINLLVPICIIEVLKNYVDKELGLKFPNDIMVKVNDNYKKLGGILTELTDD-----YMIIGIGINVNNQ---IRNEIREIAISLKEITGKELDKVEILSNFLKTFESYLEKLKNKEIDDYEILKKYKKYSITIGKQVKILLSNNEIITGKVYDIDFDGI-VLGTEKGIERIPSG-----

General information: TITO was launched using: RESULT: Template: 2EJ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1083 -65494 -60.47 -293.70 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -60.47 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_2EJ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EJ9-query.scw PDB file : Tito_Scwrl_2EJ9.pdb: