Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPE---AAEAGLKRLKKQLARD--AEKGKRTETEVKSVINRISISQTLEEAEHA-DIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSSLPITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATSEETALDVMALAEKMGKTAVEVNDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTCLSIMEVLHSGLGDSK-YRPCPLLRKYVKAGWLGKKSGRGFYDYEEKTS 1LSO Chain:B ((15-302)) --VKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLRKVAKKKFAENPKAGDEFVEKTLSTIATSTDAASVVHSTDLVVEAIVENLKVKNELFKRLDKFAAEHTIFASNTASLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK----

General information: TITO was launched using: RESULT: Template: 1LSO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1377 -25486 -18.51 -90.70 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -18.51 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1LSO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LSO-query.scw PDB file : Tito_Scwrl_1LSO.pdb: