Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTHLQAKATLHNGVEMPWFGLGVFQVEEGSELVNAVKTAIVHGYRSIDTAAIYGNEAGVGEGIREGIEEAGISREDLFITSKVWNADLGYEETLAAFETSLSKLGLDYLDLYLIHWP------------------VEGK--YKEAWRALETLYKEGRIKAIGVSNFQIHHLEDLMTAA--EIKPMINQVEFHPRLTQKELIRYCQNQGIQMEAWSPL----------MQGQLLDHPVLADIAQTYNKSVAQIILRWDLQHGIITIPKSTKEHRIKENASVFDFELTQDDMNRIDALNENLRVGPDPDNFDF 1ZUA Chain:X ((1-298)) ---HMATFVELSTKAKMPIVGLGTWKSPLG-KVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWR----------

General information: TITO was launched using: RESULT: Template: 1ZUA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1376 -48804 -35.47 -183.47 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain X : 0.79 3D Compatibility (PKB) : -35.47 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_1ZUA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZUA-query.scw PDB file : Tito_Scwrl_1ZUA.pdb: