Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQEKERILKLVEEGKLTAQEALTLIEKLDSDYKEKEEKITALSVHVHDEEEPFTTAKKESGKPSLGAKLFDWIDSAVKKVKEVDLDLNFGHAYDVQHIFQFKDTDFSSVELQIANGSVNIVPWE-DDDIRAECQAKVYRADSQDAARHAFLQHIECEIKGNKFFIRTEKKT--MKTNVTLYIPQKEYDKIRVKLFNGPVRGEH-LHVKEFSAKT-TNGVLSFS-YLTAEKAIAE-TANGQIKLASHSCGTIEAE-TINGLIDLR-GKSESIDVQ-SFNGNIAINVTESDCRSIYAK-TTTGNVELAIPDDLAVKAELKSNLGTLSHELMDVEMLKEKNDTIQKEMMFTSNQAHDQNITVFSESLTGAIKLKYSQR 4OPW Chain:A ((7-224)) ------------------------------------------------------------------------------------------GNGKLSTKKITI--DDFNAIKFDG-VIDFNYEQSESTPHIEITVDE-------------NLHPYVNIDIQDRVLTVGFKGAKVDHFTKFIVKTNSKWLKEVKAS-GNANFIANSPLKGDELKINANSNCLVQLKQKVEVGKLDLNVSGSANMVVNELKTDKLECSINGSGTINLKAGNAEEADYSITTDGEIMAFGVA--VPEVNCKITGKGSAQIHPTDNL--KATI-VGKGNIRYKGP----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OPW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1239 54239 43.78 262.02 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 43.78 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_4OPW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OPW-query.scw PDB file : Tito_Scwrl_4OPW.pdb: