Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2ORV Chain:B ((18-186)) ------RGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKD---------------MEALPACLLRDVAQE-ALGVAVIGIDEGQFFP-DIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRL------GTEKEVEVIGGADKYHSVCR-----------

General information: TITO was launched using: RESULT: Template: 2ORV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 825 -115918 -140.51 -747.86 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -140.51 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_2ORV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ORV-query.scw PDB file : Tito_Scwrl_2ORV.pdb: