Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKRTAFVMGASQGIGKAIALKLADQHFSLVINSRNLDNIESVKEDILAKHPEASVIVLAGDMSDQHTRAGIFQKIESQCGRLDVLINNIPGGAPDTFDNCNIEDMTATFTQKTVAYIDAIKRASSLMKQNEFGRIINIVGNLWKEPGANMFTNSMMNAALINASKNISIQLAPHNITVNCLNPGFIATDRYHQFVENVMKKNSISKQKAEEQIASGIPMKRVGSAEETAALAAFLASEEASYITGQQISADGGSMKSI 4J2H Chain:A ((10-257)) LSGRRALVTGASRGIGQSIAVALAEAGAHVAVTARTVEGLAETRALIEK--TGRRAVALAQDVRDVEACASVTRAAAEGLGGLDILVNNAGFENVRPSFDVDEALWDTIVSTNLKGAFFCAQAAGRIMADANGGAIVNLCSLTSYVGIPTAVPYGASKSGLLGVTRALATEWAAHNIRVNAIAPGYFRTAMTAGFYED---------EDWQSRMLEKIPQRRFGKESDIGGVAVFLCSDAAAYITGHCIPADGGYLASI

General information: TITO was launched using: RESULT: Template: 4J2H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1408 -138442 -98.32 -558.23 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -98.32 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_4J2H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J2H-query.scw PDB file : Tito_Scwrl_4J2H.pdb: