Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGEEKLNIVGTLAKRMVEKAGVPIDIHLTLDRRKALKDADFVTTQFRVGLLQARAKDERIPLKYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALLRYSNLKKVVGLCNVPIGIKMGVAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIEAMGDPKNAMTMKNISGAEWEPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRAEVVQKVEKELFELYKDPNLAIK-PPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTINNGAIASIPDDSAVEVNCVMTKTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDYQTALLAMTINPLVPSDTVAKQILDEMLEAHKAYLPQFFNKIEA 1UP7 Chain:H ((3-414)) ----MRIAVIGGGSSYTPELVKGLLDISEDVRIDEVIFYDIDE--EKQKIVVDFVKRLV-KDRFKVLISDTFE--GAVVDAKYVIFQFRPGGLKGRENDEGIPLKYGLIGQETTGVGGFSAALRAFPIVEEYVDTVRK-TSNATIVNFTNPSGHITEFVRNYLEYEKFIGLCNVPINFIREIAEMFSARLEDVFLKYYGLNHLSFIEKVFVKGEDVTEKVFENL-----KL-------EDFPTWFYDSVRLIVNPYLRYYLMEKKMFK----KISTHELRAREVMKIEKELFEKYRT---AVEIPEELT-RGGSMYSTAAAHLIRDLETDEGKIHIVNTRNNGSIENLPDDYVLEIPCYVRSGRVHTLSQGKGDHFALSFIHAVKMYERLTIEAYLKRSKKLALKALLSHPLGPDVEDAKDLLEEILEANREYV---------

General information: TITO was launched using: RESULT: Template: 1UP7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 2282 -194998 -85.45 -483.86 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain H : 0.80 3D Compatibility (PKB) : -85.45 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_1UP7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UP7-query.scw PDB file : Tito_Scwrl_1UP7.pdb: