Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNETPFSKNAEMYRDEKVFAEGEDLGLMIKTAECRAEHRVLDIGAGAGHTALAFSPYV-QECIGVDATKEMVEVASSFAQEKGV-ENVRFQQGTAESLPFPDDSFDIITCRYAAHHFSDVRKAVREVARVLKQDGRFLLVDHYAPEDPVLDEFVNHLNRLRDPSHVRESSLSEWQAMFSANQLAYQDIQKWNLPIQYDSWIKRGGTPADREKQIITHLNHASDEARDTFCITLNQNGQPISFCLKAILIQGIKR 5EPE Chain:A ((11-185)) -----------ESAHRIHNPFTPEKLATLGAALRLEAGARVLDLGSGSGEMLCTWARDHGIVGTGIDLSQLFTEQAKRRAEALGVAGQVKFIHGDAAGY-VSDEKVDVAACVGASWIAGGVAGTITLLAQSLEPGGIILMGEPFWRKLPTTEAVAKAC---HANTISDFLLLPEFLASFRKLGYDVVEMV-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EPE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 843 -87382 -103.66 -505.10 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -103.66 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_5EPE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EPE-query.scw PDB file : Tito_Scwrl_5EPE.pdb: