Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASQQQIQLARRP--QGIPVHEDFRFETIPVPEP-KQGEVLVKTLYVSVDPYMRGRM-QDTKSYVEPFALDKALSGGVIAEVVSDGNH--LKKGDIVIG-NLSWQEFSAVSESALRKIDTSLAPA--SAYLGILGMTGLTAYFGLLDIGRPKEG--ETVVVSGAAGAVGSTVGQIAKIKG-ARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAE-SEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS 2ZB8 Chain:A ((10-352)) -----QRVVLNSRPGKNGNPVAENFRMEEVYLPDNINEGQVQVRTLYLSVDPYMRCRMNEDT---ITPWQLSQVVDGGGIG-IIEESKHTNLTKGDFVTSFYWPWQTKVILDGNSLEKVDPQLVDGHLSYFLGAIGMPGLTSLIGIQEKGHITAGSNKTMVVSGAAGACGSVAGQIGHFLGCSRVVGICGTHEKCILLTSELGFDAAINYK-KDNVAEQLRESCPAGVDVYFDNVGGNISDTVISQMNENSHIILCGQISQYNKDVPYPPPLSPAIEAIQKERNITRERFLVLNYKDKFEPGILQLSQWFKEGKLKIKETVINGLENMGAAFQSMMTGGNIGKQIVCIS---

General information: TITO was launched using: RESULT: Template: 2ZB8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1924 -195053 -101.38 -598.32 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -101.38 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_2ZB8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZB8-query.scw PDB file : Tito_Scwrl_2ZB8.pdb: