Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGNEPHGGVLINRCDPACHFEGCACQA---------ELDQLALSDLELIAIGGYSPLTGFLGEKDYHSVVKEMRLANGLPWSLPITLPVGEKTARQLSAGDHVKLV-KDGVTYGMITVTDIYQPDKTQEALSVFKTNDPAHPGVKKLLAR-PDYYIGGPITV-SSLPDKSFEQFYATPAETRAAFQKLGWKTIVGFQTRNPVHRAHEYIQKTALETV-DGLLLHPLVGETKSDDIPSDIRMESYQALLNHYYPKDRVMLSVFPAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVGS------YYGTYDAQNIFQSFTEEELGIKPLFFEHSFYCRKCGNMGTSKTCPHSPRDHIHLSGTKVRELLRQGKKPPKEFSRPEVAAVLIK---GLHQQPVAIK----QNSGELQ----------------------------------------------------------------------------------------------------- 1G8F Chain:A ((2-511)) ---PAPHGGILQDLIARDALKKNELLSEAQSSDILVWNLTPRQLCDIELILNGGFSPLTGFLNENDYSSVVTDSRLADGTLWTIPITLDVDEAFANQIKPDTRIALFQDDEIPIAILTVQDVYKPNKTIEAERVFRG-DPEHPAISYLFNVAGDYYVGGSLEAIQLPQHYDYPGLRKTPAQLRLEFQSRQWDRVVAFQTRNPMHRAHRELTVRAAREANAKVLIHPVVGLTKPGDIDHHTRVRVYQEIIKRY-PNGIAFLSLLPLAMRMSGDREAVWHAIIRKNYGASHFIVGRDHAGPGKNSKGVDFYGPYDAQELVESY-KHELDIEVVPFRMVTYLPDEDRYAPIDQIDTTKTRTLNISGTELRRRLRVGGEIPEWFSYPEVVKILRESNPPRPKQGFSIVLGNSLTVSREQLSIALLSTFLQFGGGRYYKIFEHNNKTELLSLIQDFIGSGSGLIIPDQWEDDKDSVVGKQNVYLLDTSSSADIQLESADEPISHIVQKVVLFLEDNGFFVF

General information: TITO was launched using: RESULT: Template: 1G8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1954 -179303 -91.76 -468.15 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -91.76 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_1G8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G8F-query.scw PDB file : Tito_Scwrl_1G8F.pdb: