Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MSELLATYLLT-EPGADTEKKAEQIATGLTVGSWTDL-PLVKQEQMQKHKGRVIKVEEREGTAASEKQAVITIAYPEINFSQD---IPALLTTVFGKLSLDGKIK---LIDLHFSEAFKRALPGPKFGVYGIRKLLGEFE-RPLLMSIFKGVIGRDLSDIKEQLRQQALGGVDLIKDDEIFFETGLAPFETRIAEGKQILKETYEQTGHKTLYAVNLTGR-TADLKDKARRAAELGADALLFNVFAYGLDVMQGLAED-PEIPVPIMAHPAVSGAFTSSPFYGFSHALLLGKLNRYCGADFSLFPS---PYGSVALPRADALAIHEECVREDA-----FNQTF-------AVPSAGIHPGMVPLLMRDFGIDHIINAGGGVHGHPNGAQGGGRAFRAIIDAVLEAQ--PIDEKAEQCKDLKLALDKWGKAEAV 1GEH Chain:A ((12-443)) YVDKGYEPSKKRDIIAVFRVTPAEGYTIEQAAGAVAAESSTGTWTTLYPWYEQERWADLSAKAYDFHDMG-----DGSWIVRIAYPFHAFE--EANLPGLLASIAGNIFGMKRVKGLRLEDLYFPEKLIREFDGPAFGIEGVRKMLEIK-DRPIYGVVPKPKVGYSPEEFEKLAYDLLSNGADYMKDDENLTSPWYNRFEERAEIMAKIIDKVENETGEKKTWFANITA-DLLEMEQRLEVLADLGLKHAMVDVVITGWGALRYIRDLAADYGLAIHGHRAMHAAFTR-NPYHGISMFVLAKLYRLIGIDQLHVGTAEG---GKWDVIQNARILRESHYKPDENDVFHLEQKFYSIKAAFPTSSGGLHPGNIQPVIEALGTDIVLQLGGGTLGHPDGPAAGARAVRQAIDAIMQ--GIPLDEYAKTHKELARALEKWGHVTP-

General information: TITO was launched using: RESULT: Template: 1GEH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2081 -204323 -98.19 -525.25 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -98.19 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_1GEH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GEH-query.scw PDB file : Tito_Scwrl_1GEH.pdb: