Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHLTTMSELSTEEIKDLLQTAQELKSGKTDNQLTGKFAANLFFEPSTRTRFSFEVAEKKLGMNVLNL-DGTSTSV-QKGETLYDTIRTLESIGVDVCVIRHSEDEYYEELVSQV--NIPILNAGDGCGQHPTQSLLDLMTIYEEFNTFKGLTVSIHGDIKHSRVARSNAEVLTRL-GARVLFSGPS---------EWQDEEN-TFGTYVSMDEAVESSDVVMLLRIQNERHQSAVSQEGYLNKYGLTVERAERM---KRHAIIMHPAPVNRGVEIDDSLVESEKSRIFKQMKNGVFIRMAVIQRALQTNVKRGEAAYVISH 1EKX Chain:B ((8-309)) -KHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVI-STYVDAIVMRHPQ-EGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKER----LDPSEYANVKAQFVLRASDLHNAKANMKVLHPLP--RVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDL-----------

General information: TITO was launched using: RESULT: Template: 1EKX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1485 -140871 -94.86 -496.02 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -94.86 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_1EKX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EKX-query.scw PDB file : Tito_Scwrl_1EKX.pdb: