Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRHKATIEGLVMKERYYSHRAPGTERWITQPVCKVTRTEPIFEGYI-D-----IEPIEIGGKVYIPGLNE---Y-VIVTD---------RQRNIHNEWTYQTDRVIKTIIDEKSLKECEEHNNKKAKNNDTQNQRQIKTSWWQRLTKKD 4DOV Chain:A ((13-95)) ------------------------TFSWVGRPLPNRKQFQQMYREICMKINDGSEIHIKVGQFVLIQGEDNKKPYVAKLIELFQNGAEVPPKKCARVQWFVRFLEIP-------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DOV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 174 -3359 -19.30 -52.48 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -19.30 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_4DOV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DOV-query.scw PDB file : Tito_Scwrl_4DOV.pdb: