Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRTGDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGVGPSKAEAIIAYREENGRFQTIEDITK--------VSGIGEKSFEKIKSSITVK----- 2I5H Chain:A ((16-195)) -----------------EDYAYVLDFMPYGHPDDKRPIHRREPLAQVVGERNFT---LLEVSIRKGKQPLVMDRVYIGKGERDVVYKIKRRLRYEDLTPAAKTELPYVIEHIIKQDEKKYVDFFNDSI-------------------TTRMHQLELLPGVGKKMMWAIIEERKK-RPFESFEDIAQRVKGIQRPEKLIVSRIIYEIKNPQTKYKLFTA

General information: TITO was launched using: RESULT: Template: 2I5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 700 -26916 -38.45 -163.12 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -38.45 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_2I5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I5H-query.scw PDB file : Tito_Scwrl_2I5H.pdb: