Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLQPLLAKKIIAEVKKMYEREVIIVNTDGLIMAGTNDERVGQF-HEGALIC----------AKERRSVIITKEDETRLKGVKAGI---NLPVFFDHDVIAVFGLT-GEPAE-IQPFGELLRKMTELFIKESRHLE-QSQWRERMLES--FMIDWLQLKEWSPSFLEKAQLLGVDLSSRRQMILIQGYEWSPHDIEQMA-RSWKSSYPADLFIRWGNERILINHEVPQHEQRDRLLRKILHICSFANTASSQYTAAGAGRAVASSSLTDSYEQAEKALAVSLK-RKTPI-FEEDLKLDMC-LTEISPGTRNEFPQRVLGKALEHQELMNTIRTFFHHDLSLKQTAEDMHIHINTLRYRLAKAEQLTGLRFD-RTEDVVTMYVALYFLDQDTK 4OVY Chain:A ((1-305)) ---EEPLPSWNDSAARRAILEYVKSVTTEGSPRFVPVSERIVTFDNDGTLWCEQPMYVQLAFALDRVRLLADKHPEWRTEEPFRAVIEKDLPALAKLGAKGLTELTMATHAGMT---DDEFENIVTEWIRKARHPKFHRPYTECVYQPMLELLAFLRQHEF-------------------KTFIVSGAGIEF-------MRPW-----AKEVYGIPPEQVIGSSVKLKYELRD-----------------------GKPVLVRLAELNFIDDQAGKPVGIRQVIGRRPVMAVGNSDGDYEMLEYVTSG-----PANGLG-------------LIVHHTDAVREFAYDRQSPFGRLDRALTDATSKGWIVIDMQRDWKVIFPES---------

General information: TITO was launched using: RESULT: Template: 4OVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1406 13405 9.53 47.70 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.53 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.218

(partial model without unconserved sides chains): PDB file : Tito_4OVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OVY-query.scw PDB file : Tito_Scwrl_4OVY.pdb: