Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNTELELLRQKADELNLQILKLINERGNVVKEIGKAKEAQGVNRFDPVRERTMLNNIIENNDGPFENSTIQHIFKEIFKAGLELQEEDHSKALLVSRKKKPEDTIVDIKGEKIGDGQQRFIVGPCAVESYEQVAEVAAAAKK-----QGIKILRGGAFKP--RTSPYDFQGLGVE-GLQILKRVADEFDLAVISEIVTPAHIEEALDYIDVIQIGARNMQNFELLKAAGAVKKPVLLKRGLAATISEFINAAEYIMSQGNDQIILCERGIRTYETATRNTL--DISAVPILKQETHLPVFVDVTHST----GRRDLLLPTAKAALAIGADGVMAEVHPDPSVALSDSAQQMAIPEFEKWLNELKPMVKVNA 1PCW Chain:B ((7-244)) -------------------------------------------------------------------------------------------------------------------------IAGPCAIESEELLLKVGEEIKRLSEKFKEVEFVFKSSFDKANRSSIHSFRGHGLEYGVKALRKVKEEFGLKITTDIHESWQAEPVAEVADIIQIPAFLCRQTDLLLAAAKTGRAVNVKKGQFLAPWDTKNVVEKLKFGGAKEIYLTERGT----TFGYNNLVVDFRSLPIMKQWAK--VIYDATHSVQLPG-MREFIFPLIRAAVAVGCDGVFMETHPEPEKALSDASTQLPLSQLE--------------

General information: TITO was launched using: RESULT: Template: 1PCW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1145 15839 13.83 73.33 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : 13.83 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1PCW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PCW-query.scw PDB file : Tito_Scwrl_1PCW.pdb: