Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDMKHTLLEALGIEIVENTAERCVAVMPVDHRTVQPFGYLHGGASVALAETAASAGAQNLIDHTTQACVGLEINANHLKSVKEGTVKAIAEPVHIGRTTIVYHIHIYDEQERLICISRCTLAVIKK 4K4B Chain:F ((38-136)) ------------------------ATMPVDSRTKQPFGLLHGGASVVLAESIGSV-AGYLCTEGEQKVVGLEINANHVRSAREGRVRGVCKPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL--

General information: TITO was launched using: RESULT: Template: 4K4B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 458 -60419 -131.92 -610.29 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain F : 0.79 3D Compatibility (PKB) : -131.92 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_4K4B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K4B-query.scw PDB file : Tito_Scwrl_4K4B.pdb: