Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDV-SHK--KLTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINNAGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKG--TENGRIYDIKEFL 2D1Y Chain:A ((6-242)) GKGVLVTGGARGIGRAIAQAFAREGALVALCDLRPEGKEVAEAIGGAFFQVDLEDERERVRFVEEAAYA----LGRVDVLVNNAAIAAPG-SALTVRLPEWRRVLEVNLTAPMHLSALAAREMRKV-GGGAIVNVASVQGLFAEQENAAYNASKGGLVNLTRSLALDLA--PLRIRVNAVAPGAIATEAVLEAIA--------RRDWEDLHALRRLGKPEEVAEAVLFLASEKASFITGAILPVD---

General information: TITO was launched using: RESULT: Template: 2D1Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1259 -41473 -32.94 -185.15 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -32.94 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_2D1Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D1Y-query.scw PDB file : Tito_Scwrl_2D1Y.pdb: