Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIAVINGGTRSGGNTDVLAEKAVQGFDAEHIYLQKYPIQPIEDLRHAQGGFRPVQDDYDSIIERILQCHILIFATPIYWFGMSGTLKLFIDRWSQTLRDPRFPDFKQQMSVKQAYVIAVGGDNPKIKGLPLIQQFEHIFHFMGMSFKGYVLGEGNRPGDILRDHQALSAASRLLKRSDAI 3GFS Chain:A ((3-132)) --MLVINGTPRKHGRTRIAASYIAALYHTDLIDLSEFVLPVFNGE--AEQ---SELLKVQELKQRVTKADAIVLLSPEYHSGMSGALKNALDFLSS-----------EQFKYKPVALLAVAGGGD--GGINALNNMRTVMRGVYANVIPK-------------------------------

General information: TITO was launched using: RESULT: Template: 3GFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 617 -94093 -152.50 -723.79 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -152.50 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_3GFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GFS-query.scw PDB file : Tito_Scwrl_3GFS.pdb: