Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAILVTGGAGYIGSHTCVELLNSGYEIVVLDNLSNSSAEALNRVKEITGKDLTFYEADLLDREAVDSVFAENEIEAVIHFAGLKAVGESVAIPLKYYHNNLTGTFILCEAMEKYGVKKIVFSSS--------ATVYGVPETS---PITEDFPLGATNPYGQTKLMLEQILRDLHTADNEWSVALLRYFNPFGAHPSGRIGEDPNGIPNNLMPYVAQVAVGKLEQLSVFGNDYPTKDGTGVRDYIHVVDLAEGHVKALEKVLNSTGADAYNLGTGTGYSVLEMVKAFEKVSGKEVPYRFADRRPGDIATCFADPAKAKRELGWEAKRGLEEMCADSWRWQSSNVNGYKSAE 4ZRM Chain:B ((4-298)) MNILVTGGAGFIGSHVVDKLIENGYGVIVVDNLSSGKVENLNR-------NALFYEQSIEDEEMMERIFSLHRPEYVFHLAAQASVAISVREPARDAKTNIIGSLVLLEKSIKYGVKKFIFSSTGGAIYGENVKVFPTPETEIPHPIS---------PYGIAKYSTEMYL-EFFAREYGLKYTVLRYANVYGPR------QDPYGEAGVVAIFTERMLRG--EEVHIFG------DGEYVRDYVYVDDVVRANLLAMEKGDN----EVFNIGTGRGTTVNQLFKLLKEITGYDKEPVYKPPRKGDVRKSILDYTKAKEKLGWEPKVSLEE--------------------

General information: TITO was launched using: RESULT: Template: 4ZRM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1646 -73457 -44.63 -258.65 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -44.63 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_4ZRM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRM-query.scw PDB file : Tito_Scwrl_4ZRM.pdb: