Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRITINIITMFI---AAAVISLTGTAEAAEKQQQSPANVTLTDQQKKEIEQLEAEILKKRKDVISKYVQYGILPKERGEHIKNHLDKHFEMMKQNGFVPKHHPHPHKFEKRH 2MQ8 Chain:A ((1-112)) MLTVEVEVKITADDENKAEEIVKRVIDEVEREVQKQYPNATITRTLTRDDGTVELRIKVKA-DTEEKAKSIIKLIEERIEEELRKRDPNATITRTVRTEVGSSWSLEHHHHHH

General information: TITO was launched using: RESULT: Template: 2MQ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 440 -20790 -47.25 -190.73 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -47.25 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_2MQ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MQ8-query.scw PDB file : Tito_Scwrl_2MQ8.pdb: