Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMTKKDTNISTQQPLWLQGYIDSP---------EKQNQLYKKTLKILIFSQIF-----GGAGLGA-------GITVGALLAQDMIGSENVA---GIPTALFTFGSAVAALLIGASSQRFGRRAGLAGGFLIGGLGAIGVIIAALINSV-------ALLFVSLLIYGAGMASNLQVRYAGTDLA----NEKQRATAASMALVSTTLGAVVGPNLVNTMGEFADSIGVPNLAGPFIMSGAAFIIAGIILLIFLRPDPLF----VSTAIANAEKKDDKVQIGGSLKNPAI--DKKGIMVGAVIMILAQLIMTAIMTMTPVHMGHHGH---GLSEVGLVI------GLHIAAMYLPSPLTGLLVDKFGR----TTMAIASGATLLAAGLVAAIAPAD-SLSLLI--LALVLLGVGWNFGLLTGTALIIDSTHPSLRAKTQGTFDVLLALSGAAGGALSGMVVAHSS-------YTILSISGAVLSLLLIPVVIWYFRRIQEKA 4IKW Chain:A ((3-494)) ---SIDKQQIAASVPQ--RGFFGHPKGLFTLFFTEFWERFSYYGMRAILVYYMYYEVSKGGLGLDEHLALAIMSIYGALVYMSGIIGGWLADRVFGTSRAVFYGGLLIMAGHIALAIPGGVAALFVSMALIVLGTGLLKPNVSSIVGDMYKPGDDRRDAGFSIFYMGINLGAFLAPLVVGTAGMKYNFHLGFGLAAVGMFLGLVVFVATRKKNL-GLAGTYVPNPLTPAEKKKAAAIMAVGAVVIAVLLAILIPNGWFTVETFISLVGILGIIIPIIYFVVMYRSPKTTAEERSRVIAYIPLFVASAMFWAIQEQGSTILANYADKRTQLDVAGIHLSPAWFQSLNPLFIIILAPVFAWMWVKLGKRQPTIPQKFALGLLFAGLSFIVILVPGHLSGGGLVHPIWLVLSYFIVVLGELCLSPVGLSATTKLAPAAFSAQTMSLWFLSNAAAQAINAQLVRFYTPENETAYFGTIGGAALVLGLILLAIAPRIGRLMKG--

General information: TITO was launched using: RESULT: Template: 4IKW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1914 -252471 -131.91 -589.88 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -131.91 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_4IKW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IKW-query.scw PDB file : Tito_Scwrl_4IKW.pdb: