Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFEQSHVLKELPKQFFASLVQKVNRKLAEGHDVINLGQGNPDQPTPEHIVEEMKRAVADPENHKYSSFRGSYRLKSAAAAFYKREYGIDLDP-ETEVAVLFGGKAGLVELPQCLLNPGDTILVPDPGYPDYWSGVTLAKAKMEMMPLVKDRAFLPDYSSITAEIREQAKLMYLNYPNNPTGAVATSEFFEDTVRFAAENGICVVHDFAYGAVGFDGCKPLSFLQTEGAKDIGIEIYTLSKTYNMAGWRVGFAVGNASVIEAINLYQDHMFVSLFRATQEAAAEALLADQTCVAEQNARYESRRNAWITACREIGWDVTAPAGSFFAWLPVPEG--YTSEQFSDLLLEKANVAVAAGNGFGEY--GEGYVRVGLLTSEERLKEAAYRIGKLNLFTQKSIDKTL 1U08 Chain:A ((9-385)) -----QSKLPQLGTTIFTQMSALA-----QQHQAINLSQGFPDFDGPRYLQERLAHHVA-QGANQYAPMTGVQALREAIAQKTERLYGYQPDADS-DITVTAGATEALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQP-PHFRVDWQEFAALLSERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASVLAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLALADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDDVEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQ-------------

General information: TITO was launched using: RESULT: Template: 1U08.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2148 -240668 -112.04 -646.96 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -112.04 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_1U08.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U08-query.scw PDB file : Tito_Scwrl_1U08.pdb: