Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNEKRLLEEFLELVQIDSETKH----------EAEICKVLKRKFSDL---GVDVKEDDTMDITGHGAGNLICTLKGTKQTDTIYFTSHMDTVVPGNGV----KPV---VENGYVKTDGTTILGADDKAGLAAMFEAIKVLKEENIEHGTIEFIITVGEESGLIGAKALDRSM---I-TASYGYALDSDGKV---GNIIVAAPTQAKVRAAIF--GKTAHAGVEP-EKGISAITIASKAISKMPLGR---I----------------------------------------------------DEETTANIGRFEGG----TQTNIVCDEVHILAEARSLVPEKMEAQVQKMKAAFEEAAADMGGRAEVEIEV-MYPGFKYQDGDQ-VVEIAKKAAAK-IGRPSELQTSGGGSD-ANVIAG----H-GIPTVNLAVGYEQIHTKNEKMPIEELVKTAEMVVAIIEEAAK 3PFE Chain:A ((18-470)) ----EEILPSLCDYIKIPNKSPHFDAKWEEHGYMEQAVNHIANWCKSHAPKGMTLEIVRLK----NRTPLLFMEIPGQ-IDDTVLLYGHLDKQPEMSGWSDDLHPWKPVLKNGLLYGRGG----ADDGYSAYASLTAIRALEQQGLPYPRCILIIEACEESGSYDLPFYIELLKERIGKPSLVICLDSGAGNYEQLWMTTSLRGNLVGKLTVELINEGVHSG-SASGIVADSFRVARQLISRIEDENTGEIKLPQLYCDIPDERIKQAKQCAEILGEQVYSEFPWIDSAKPVIQDKQQLILNRTWRPALTVTGADGFPAIADAGNVMRPVTSLKLSMRLPPLVDPEAASVAMEKALTQNPP-YNAKVDFKIQNGGSKGWNAPLLSDWLAKAASEASMTYYDKPAAYMGEGGTIPFMSMLGEQFPKAQFMITGVLGPHS-NAHGPNEFLHLDMVKKLTSCVSYVLYSFSQ

General information: TITO was launched using: RESULT: Template: 3PFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1562 9842 6.30 27.72 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 6.30 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_3PFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFE-query.scw PDB file : Tito_Scwrl_3PFE.pdb: