Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIFAHRGASGQFPENTMLAFEKGIEAGADGIELDVQLTKDGRIVVIHDERLNRTTSLKGFVKDTAYDEVKTANAAAGHDQ--------AYSDIKVPLLEDVLSWAVKKDFLINIELKNSVIRYEGMEEKVL-EAVKRFNV------EDRVILSTFNHDSLALCARLAPHIERAVLTSD-VLY---QADRY----------I-----ASIPASGYHPKIN----SPGVTDEVLKKMR-NGLIKVRPYTVNRPEDMKRLIEAGADGMFTDFPEKASALLKNE 4R7O Chain:A ((15-291)) KFLNIAHRGASGHAPEHTFASYDLVKKMKADYLELDIQLTKDGQLIAMHDTAVDRTTNGTGEVRDKTLSEIKSLDAGSWFNKAYPEKAKQEYVGQKVPTLEEIFQKYGR-SMKYYIETKSPDV-YPGMEE-KLLALLEKYNLIGQNMSSSRVMIQSFSKDSLKKIHSINKNIPLVQLLWYYPNENNEIVEWSGITHEPKRVTNDDFQEIKKYAVGIGPNLRNDNGDLIINESYMKMARQNG-LLIHPYTINEKPDMRLLMKWGATGMFTNYPDRLHTVLKE-

General information: TITO was launched using: RESULT: Template: 4R7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1157 1649 1.43 6.93 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 1.43 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_4R7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R7O-query.scw PDB file : Tito_Scwrl_4R7O.pdb: