Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLETKDLNLFLGN-KHVLKNISLSIPVRGEIIGIMGPNGAGKSSLIKSLIGEFN-ATGTKLLYNKPIQ-------QQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIGWFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDEPFVGIDFSSEKLIMTKIENLKQ-QGKLILIIHHDLSKAKQYFDRIILL-NQTLRYFGDSEEAMSVTRLNETFMSSTDCSDPSQRSNITC 3GFO Chain:A ((7-237)) ILKVEELNYNYSDGTHALKGINMNIK-RGEVTAILGGNGVGKSTLFQNFNGILKPSSGRILFDNKPIDYSRKGIMKLRESIGIVFQDPDN-QLFSASVYQDVSFGAVNMK----LPEDEIRKRVDNALKRTGIEHLKDKPTHCLSFGQKKRVAIAGVLVMEPKVLILDEPTAGLDPMGVSEIMKLLVEMQKELGITIIIATHDIDIVPLYCDNVFVMKEGRVILQGNPKEVFAEKEV---------------------

General information: TITO was launched using: RESULT: Template: 3GFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -152309 for 1745 contacts (-87.3/contact) + 2D Compatibility (PS) -23376 + (NN) -5931 + (LL) 876 1D Compatibility (HY) -12800 + (ID) 3250 Total energy: -196790.0 ( -112.77 by residue) QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_3GFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GFO-query.scw PDB file : Tito_Scwrl_3GFO.pdb: