Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAV-----DIAEAVSETVDKIKSNGDNAKAYNVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEYPIDVYDKIMNVDMRGTILMTKMMLPLMMNQGGSIVNTSSFSG----QAEDLYRSGYNAAKGAVINFTKSIAIEYGRDGIRSNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLGRLGKPEEVAKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE 4I5E Chain:D ((16-259)) --RLLNKTAVITGGNSGIGLATAKRFVAEGAYVFIVGRRRKELEQAAAEI-------GRNVTAVKADVTKLEDLDRLYAIVREQRGSIDVLFANSGAIEQK-TLEEITPEHYDRTFDVNVRGLIFTVQKALPLLRD-GGSVILTSSVAGVLGLQAHDTY----SAAKAAVRSLARTWTTELKGRSIRVNAVSPGAIDTPIIENQVSTQEE--ADELRAKFAAATPLGRVGRPEELAAAVLFLASDDSSYVAGIELFVDGGL--------------------

General information: TITO was launched using: RESULT: Template: 4I5E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -94207 for 1993 contacts (-47.3/contact) + 2D Compatibility (PS) -25264 + (NN) -7306 + (LL) 1892 1D Compatibility (HY) -14400 + (ID) 4850 Total energy: -144135.0 ( -72.32 by residue) QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_4I5E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I5E-query.scw PDB file : Tito_Scwrl_4I5E.pdb: