Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVEDDHSISEMV-DHYLTKEGFGIVHAF-DGEEGIRLFQQGSYDLVLLDIMLPKLNGMDFLKIIREKSNIPVLMISAKDGDVDKALGLGFGADDYIAKPFSMIELTARVKAAIRRATQYSAEEPAVNKVIRIHQLAIDIDNVSVLKNGEPLQLTSTEWQLLCLFASNPKKVFTKEQIYRSVWNEEYFDDQNIINVHMRRLREKIEDDPSSPQYIKTLWGIGYKLGEF 5DCL Chain:B ((10-120)) -IVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEIIAFQP---DLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFISKPFSLAVLDAKLTAILR-----------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5DCL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 441 -77578 -175.91 -711.72 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -175.91 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_5DCL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DCL-query.scw PDB file : Tito_Scwrl_5DCL.pdb: