Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVEELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTD--HFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITRLISLTRGV-----KCRPEQMIIGAGTQVLMQLLTE-LLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKG---MSIAEIT---RQQPDVLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLL-PGLRISYMVLPP-E-----LLRAY-KQRGYDLQTCSSLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSGEVTVKGANAGLHFVTEFDTRR-----TEQDILSHAA-GLQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAVYGHKKIPVTGD 4IX8 Chain:A ((65-443)) --------------------------------------------------------------------------------------------------------STKSNLRLSIGDPTVDGNLKTPDIVTEAMVDVVRS--GKFNGYPPTVGADNLRQVVSTYWRRFCQTKSRQEALKWENVIITSGVSQAIVLALTALCNEGDNILVCAPSFPHYKSVCDSYGIECRYYYLDPSKSWECDLRAAAGMVDSHTKAFVII-NPSNPCGSNFSRAHVSDIIDFCQQ-HQIPLISDEIYAEMVLNNGIFTSVADFDTNVPRLILGGTAKYQVCPGWRVGWSILIDPMNVAGDWAVGMERLTQ-LIAGVNSICQEAIARTLLKCP-TECTEHIVTQLEAGAKVYARLL-EHD-IGISMEAPQASMFVMLKLNLSYFQDLKSDMEFYEKLLDEENVQVLPGEIFGM--------SGFLRATVSRP-SAVLNEAVDRIIEFCERHK-------

General information: TITO was launched using: RESULT: Template: 4IX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1965 -228357 -116.21 -650.59 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -116.21 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_4IX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IX8-query.scw PDB file : Tito_Scwrl_4IX8.pdb: