Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFAVIVLPGSNCDIDMYHAVKDELGHEVEYVWHEE--TSL--DGFDGVLIPGGFSYGDYLRCGAIARFANIMPAVKQAAAEGKPVLGVCNGFQILQELGLLPGAMRRNKDLKFICRPVELIVQNDETLFTASYEKG------ESITIPVAHGEGNFYCDDETLATLKENNQIAFTYGSNINGSVSDIAGVVNEKGNVLGMMPHPERAVDELLGSADGLKLFQSIVK-NWRETHVTTA 1GPM Chain:A ((8-225)) HRILILDFGSQY-TQLVARRVREL-GVYCELWAWDVTEAQIRDFNPSGIILSGGPESTTEE---NSP------RAPQYVFEAGVPVFGVCYGMQTMAMQL---GGHVEAS-NEREFGYAQVEVV-NDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIP---------SDFITVAS--TESCP---FAIMANEEKRFYGVQFHPEVTHTR-QG----MRMLERFVRDICQ-------

General information: TITO was launched using: RESULT: Template: 1GPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1140 -120533 -105.73 -651.53 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -105.73 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_1GPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GPM-query.scw PDB file : Tito_Scwrl_1GPM.pdb: