Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIW----TEDASRMEAMSLDYFLSYPRLFWPKF---KELFQMKMSGSFEPNEGHLLLAELE----KQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACG---ARYDL-------------PHLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAVMH--FDTLYEKLDQADLLLVIGTSLEVAPAR-FVPEDASLIPGMKKVIINLEPTYCDSLFDMVIHQKIGEFARSLGMKK 4UU7 Chain:B ((13-258)) LTAFREHFAKAKHIAIITGAGVSAESGVPTFRGPGGFWRKWQAQDLATPEAFSRD-----PSLVWEFYHYRREVMRSKM-----PNPAHLAIAECEARLGQQGRSVVIITQNIDELHHRAGSKHVYEIHGSLFKTRCMSCGEVKANHKSPICPALDGKGAPDPNTKEARIPVELLPRCERKSCNGLLRPHVVWFGETLDSDILTAVERELEKCDLCLVVGTSSIVYPAAMFAPQVASR--GVPVAEFNMECTPATQRF-------------------

General information: TITO was launched using: RESULT: Template: 4UU7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 982 -94674 -96.41 -438.31 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -96.41 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_4UU7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UU7-query.scw PDB file : Tito_Scwrl_4UU7.pdb: