Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDKTMKDIQAEVDRYIGQFKEGYFSPLAMMARLTEELGELAREVNHRYGEKPKKATEDDKSMEEEIGDVLFVLVCLANSLDISLEEAHDRVMHKFNTRDKDRWTRKEEGK 5IE9 Chain:A ((1-103)) MEAKTMKDMQKEVDAYIGQFKEGYFSPLAMMARLTEEMGELAREVNHYYGE---------RSIEEELGDVLFVMICMANSLNIDLETAHNIVMNKFNTRDKDR--------

General information: TITO was launched using: RESULT: Template: 5IE9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 192 -19812 -103.18 -210.76 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -103.18 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_5IE9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IE9-query.scw PDB file : Tito_Scwrl_5IE9.pdb: