Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIRTITSSDYEMVTSVLNEWWGGRQLKEKLPRLFFEHFQDTSFITSEHNSMTGFLIGFQSQSDPETAYIHFSGVHPDFRKMQIGKQLYDVFIETVKQRGCTRVKCVTSPVNKVSIAYHTKLGFDIEKGTKTVNGISVFANYDGPGQDRVLFVKNI 3BLN Chain:A ((3-141)) KNVTKASIDDLDSIVHIDIDVIGNDSRRNYIKHSI---DEGRCVIVKEDNSISGFLTYDTN--FFDCTFLSLIIVSPTKRRRGYASSLLSYMLSHSPTQ---KIFSSTNESNESMQKVFNANGFIRSG---------IVENLDEGDPEIIFYTKKL

General information: TITO was launched using: RESULT: Template: 3BLN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 637 -80239 -125.96 -577.26 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -125.96 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_3BLN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BLN-query.scw PDB file : Tito_Scwrl_3BLN.pdb: