Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDPSTLMILAIVAVAIIVLAVFFTFVPVMLWISALAAGVKISIFTLVGMRLRRVIPNRVVNPLIKAHKAGLNVGTNQLESHYLAGGNVDRVVNALIAAQRANIELTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGIEVKAKARITVRANIERLVGGAGEETIVARVGEGIVSTIGSSDNHKKVLENPDMISQTVLGKGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMRARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSDEDRKS 4U5T Chain:A ((2-38)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DPDLEIRAAFLEKENTALRTEVAELRKEVGRCKNIVS-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4U5T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 9 307 34.06 8.28 target 2D structure prediction score : 0.84 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : 34.06 2D Compatibility (Sec. Struct. Predict.) : 0.84 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.949

(partial model without unconserved sides chains): PDB file : Tito_4U5T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U5T-query.scw PDB file : Tito_Scwrl_4U5T.pdb: