Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFREVIEQRYHQLLSRYIAELTETSLYQAQKFSRKTIEHQIPPEEIISIHRKVLKELYPSLPEDVFHSLDFLIEVMIGYGMAYQEHQTLRGIQQEIKSEIEIAANVQQTLLGTKVPQEEALDIGAISV--PAKQMSGDYYHFVKDKESINIAIADVIGKGIPAALCMSMIKYAMDSLPETGIHPSQVLKNLNRVVEQNVDASMFITMFYANYNMDKHQFTYASAGHEPGFYYSQKDNTFYDLEAKGLVLGISQDYDYKQFDQHLEKGDMIVLFSDGVTECRTENGFLERPDLQKLIEEHMCSSAQEMVKNIYDSLLKLQDFQLHDDFTLIVLRRKV 3ZT9 Chain:A ((3-193)) ------------------------------------------------------------------------------------------------------------------------KLEVGIYTRAREGEIACGDACLVKRVEGVIFLAVGDGIGHGPEAARAAEIAIASMESSMN--TGLVNIFQLCHRELR----GTRGAVAALCRVDRRQGLWQAAIVGNIHVKILSAKG-IITPL-ATPGILGYNYPHQLLIAKGSYQEGDLFLIHSDGIQEGAVPL------A---LLANY-RLTAEELVRLIGEKY-----GRRDDDVAVIVAR---

General information: TITO was launched using: RESULT: Template: 3ZT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1145 -167585 -146.36 -886.69 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -146.36 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_3ZT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZT9-query.scw PDB file : Tito_Scwrl_3ZT9.pdb: