Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSHYKYPLIFTAFLLIAFCLIFFSYHLIHHVRLEYPNWQGESKDQNWEAVFTKNEDAPNEYSGKLYWIGDTIEIDNTYLESLIVKKDDEVLLSSDTEIPMHDYAGGTFSDGSAKEKSVSFLERLDEHELEGHDITIEVKWKQGNHYSASQLTLNEKTLFDEKQQ 2MQ8 Chain:A ((1-112)) ------------------------MLTVEVEVKITADD------ENKAEEIVKRVIDEVEREVQKQY--------PNATITRTLTRDDGTVELRIKVKAD---------TEEKAKSI-IKLIEERIEEELRKRDPNATITRTVRTEVG-SSWSLEHHHHHH----

General information: TITO was launched using: RESULT: Template: 2MQ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 464 -26193 -56.45 -233.87 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -56.45 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.259

(partial model without unconserved sides chains): PDB file : Tito_2MQ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MQ8-query.scw PDB file : Tito_Scwrl_2MQ8.pdb: