Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MLAVNFTAFFYNLNISNLTRQVNKMKMDELEKVMIVEGKS-------------DKEKIESVLNEPMRIICTNGTISQLRLEELADELYDKDV---------------YILV---DADESGEKLRKQLKREFNEAC------HLHVDR-----------AYKEVAAAP---RHH------IASVLLRANLN-------VHTIFLERKSRGV 2A35 Chain:A ((4-215)) TPKRVLLAGATGLTGEHLLDRILSE------PTLAKVIAPARKALAEHPRLDNPVGPLAELLPQLDGSIDTAFCCLGTTIKEAGSEEAFRAVDFDLPLAVGKRALEMGARHYLVVSALGADAKSSIFYNRVKGELEQALQEQGWPQLTIARPSLLFGPREEFRLAEILAAPIAGKYHGIEACDLARALWRLALEEGKGVRFVESDELRKLGKGS

General information: TITO was launched using: RESULT: Template: 2A35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 632 8602 13.61 61.44 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 13.61 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_2A35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A35-query.scw PDB file : Tito_Scwrl_2A35.pdb: