Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLFKSFDASEKTQLICFPFAGGYSASFRPLHAFLQGECEMLAAEPPGHGTNQ-TSAIEDLEELTDLYKQELNLRPDRPFVLFGHSMGGMITFRLAQKLEREGIFPQAVIISAIQPPHIQ-R---KKVSH-LPDDQFLDHIIQ-LGG-MPAELV-ENKEVMSFFLPS----FRS----DYRALEQFE-LYDLAQIQSPVHVFNGLDDKK-CIRDAEGWKKWAKDITFHQFDGGHMFLLSQTEEVAERIFAILNQHPIIQP 3OM8 Chain:A ((19-266)) ----YRLDGAAEKPLLALSNSIGTTLHMWDAQLPALTRHFRVLRYDARGHGASSVPPGPYTLARLGEDVLELLDALEVRRAHFLGLSLGGIVGQWLALHAPQ---RIERLVLANTSAWLGPAAQWDERIAAVLQAEDMSETAAGFLGNWFPPALLERAEPVVERFRAMLMATNRHGLAGSFAAVRDTDLRAQLARIERPTLVIAGAYDTVTAASHGELIAASIAGARLVTLPAVHLSNVEFPQAFEGAVLSFLGA------

General information: TITO was launched using: RESULT: Template: 3OM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1221 -146397 -119.90 -639.29 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -119.90 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3OM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OM8-query.scw PDB file : Tito_Scwrl_3OM8.pdb: