Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHRGKGMKFVGDSRIPAEKKPNIPKDYSEYPGKTEAFWPNFLLKEWMVGAVFLIGFLVLTIVHQPPLERMADPTDTGYIPLPDWYFLFLYQLLKYEYAAGSFTVVGAMIMPGLAFGALLLAPFLDRGTERRPWKRPVAVGMMLLAISAAVFLTWQSVATHDWAKAEEQGKITKEADIDTNAEGYKVFKEQGCISCHGDNLQ---------------------------GGAAGPSLVD--SGLKPDEIKKIAVEGKG-----KMPAGVF------------------------------------KGNDKQLEELAKFISETTAK 3MK7 Chain:B ((49-197)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTALQLEGRDLYIREGCVGCHSQMIRPFRAETERYGHYSVAGESVYDHPFLWGSKRTGPDLARVGGRYSDDWHRAHLYNPRNVVPESKMPSYPWLVENTLDGKDTAKKMSALRMLGVPYTEEDIAGARDSVNGKTEMDAMVAYLQVLGT-

General information: TITO was launched using: RESULT: Template: 3MK7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 206 2044 9.92 25.87 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.52 3D Compatibility (PKB) : 9.92 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.607

(partial model without unconserved sides chains): PDB file : Tito_3MK7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MK7-query.scw PDB file : Tito_Scwrl_3MK7.pdb: