Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLSAALLMKKEDVKQLLEVRKLLEIGVASLAAEKR-TEADLERIQDALKEMGSIE--ADGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRETRKIWLFSKKTSVQRLYEEHERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ 3IHU Chain:A ((28-220)) GLELGTFVPGQRLV-ETDLVAHFGVGRNSVREALQRLAAEGIVDLQRHRGAVIRR--------------L-SLQETLDVLDVAERMTGLLARAATRGSGNQPQVQALRASVQALVAAEKAQDGETFSNARRHFYRTLLEMGDNRELRRLFPTIHMPIVHAQH-----R--L-ASLRQMRLDDYRRIATAVLAGEPDAAEAAGAAHVKNVRGAILDRQ-----

General information: TITO was launched using: RESULT: Template: 3IHU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 784 -27652 -35.27 -145.54 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -35.27 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_3IHU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IHU-query.scw PDB file : Tito_Scwrl_3IHU.pdb: