Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLLAAGIIGLLTVSIASPSFAAEKQADTNVAVLFDGSGS-MVQKTGGERKIDIAKKSVKSFAELLPKDTNLML-RVFGHAGNNKLSGKALSCSTTETIYGLH-PYEGSLFDNSLSELKPTGWTPIAKALADTRKEFEAFDADGKNVVYLITDGEETCGGDPAAEIEKLRASNVDTIVNIIG-FNFD--------------------VKGNEE-MKQAAVAGGGEYISANSADEFEQAWEKEAQK-FTE 3IBS Chain:A ((10-215)) ---------------------------KGVEVIIALDISNSMLAQDVQPSR-LEKAKRLISRLVDELDND--KVGMIVFAGDAFTQLP-------ITSDYISAKMF-----LESISPSLISKQGTAIGEAINLATRSFTP-QEGVGRAIIVITDGENH-EGGAVEAAKAAAEKGIQVSVLGVGMPEGAPIPVEGTNDYRRDREGNVIVTRLNEGMCQEIAKDGKGIYVRVDNSNSAQKAISQEISKMAKS

General information: TITO was launched using: RESULT: Template: 3IBS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 966 64889 67.17 360.49 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 67.17 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_3IBS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IBS-query.scw PDB file : Tito_Scwrl_3IBS.pdb: