Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAILVTGGAGYIGSHTCVELLNSGYEIVVLDNLSNSSAEALNRVKEITGKDLTFYEADLLDREAVDSVFAENEIEAVIHFAGLKAVGESVAIPLKYYHNNLTGTFILCEAMEKYGVKKIVFSSS-ATVYG-VPETSPITEDFPLGATNPYGQTKLMLEQILRDLHTADNEWSVALLRYFNPFGAHPSGRIGEDPNGIPNNLMPYVAQVAVGKLEQLSVFGNDYPTKDGTGVRDYIHVVDLAEGHVKALEKVLNSTGADAYNLGTGTGYSVLEMVKAFEKVSGKEVPYRFADRRPGDIATCFADPAKAKRELGWEAKRGLEE---MCADSWRWQSSNVNGYKSAE 2P5Y Chain:A ((1-308)) MRVLVTGGAGFIGSHIVEDLLARGLEVAVLDNLATGK-------RENVPKGVPFFRVDLRDKEGVERAFREFRPTHVSHQAAQASVKVSVEDPVLDFEVNLLGGLNLLEACRQYGVEKLVFASTGGAIYGEVPEGERAEETWPPRPKSPYAASKAAFEHYL-SVYGQSYGLKWVSLRYGNVYGPR------QDPHGEAGVVAIFAERVLKGL--PVTLYARKTPGDEGC-VRDYVYVGDVAEAHALALFSL-----EGIYNVGTGEGHTTREVLMAVAEAAGKAPEVQPAPPRPGDLERSVLSPLKLMAH-GWRPKVGFQEGIRLTVDHFR-------------

General information: TITO was launched using: RESULT: Template: 2P5Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1839 -74755 -40.65 -246.71 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -40.65 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_2P5Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P5Y-query.scw PDB file : Tito_Scwrl_2P5Y.pdb: