Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAGSMVALVTPFDAQGRLDWDSLAKLVDFHLQEGTNAIVAVGTTGESATLDVEEHIQVIRRVVDQVKGRIPVIAGTGANSTREAVALTEAAKSGGADACLLVTPYYNKPTQEGMYQHFRHIAEAVAIPQILYNVPGRTSCDMLPETVERLSKVPNIIGIKEATGDLQRAKEVIERVGKDFLVYSGDDATAVELMLLGGKGNISVTANVAPRAMSDLCAAAMRGDAAAARAINDRLMPLHKALFIESNPIPVKWALHEMGLIPEG-IRLPLTWLSPRCHEPLRQAMRQTGVLA 4PFM Chain:A ((2-289)) MINGSIVALITPLNSDGTVDYTSLEKLVEYHITEGTDAIVAVGTTGESATLPISEHIAVVGQTVKFASGRIPVIGGNGANATAEAIELTKAQNKLGVAAMLGVTPYYNKPSPKGLIAHYTAVAASTDIPQILYNVPGRTAVDMLPETIAQLVEVPNIIGV-DATGDVARVKQLRDLCGNDFLLYSGDDATAREFLTLGGDGVISVANNIVPKLFKLMCDAALAGDTQAAMAAEDQIKGLFSALFCEANPIPVKWAAHKMGLISQGDIRLPLTELSTEFHGLLLDAMKNARIEV

General information: TITO was launched using: RESULT: Template: 4PFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -191753 for 2551 contacts (-75.2/contact) + 2D Compatibility (PS) -31389 + (NN) -19753 + (LL) 64 1D Compatibility (HY) -30000 + (ID) 8100 Total energy: -280931.0 ( -110.13 by residue) QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_4PFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PFM-query.scw PDB file : Tito_Scwrl_4PFM.pdb: