Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIDSIDFQILQLLNKNARIQWKEIGEKIHMTGQAVGNRIKKMEDNGIIKAYSIVVDELKMGFSFTAFVFFFMN-AYMHDDLLKFIATRNEISEAHRVSGDACYLLKVTV-HSQEVLNHLLN-DLLKYG---NYQLYLSIKEVKKHYNTSLMSDE 2DBB Chain:A ((6-149)) -KLDRVDMQLVKILSENSRLTYRELADILNTTRQRIARRIDKLKKLGIIRKFTIIPDIDKLGYM-YAIVLIKSKVPSDADKVISEISDIEYVKSVEKGVGRYNIIVRLLLPKDIKDAENLISEFLQRIKNAENVEVILISEVRKFE---------

General information: TITO was launched using: RESULT: Template: 2DBB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 430 -98904 -230.01 -716.69 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -230.01 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2DBB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DBB-query.scw PDB file : Tito_Scwrl_2DBB.pdb: