Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKLSEEMKQTIMDIFEHLHANPEVSWK---EYETTSFLKQKLEDLGCRTRTFS--DCTGVVGEIGSGSPVVAVRADIDALWQEVNG-----TFRANHSCGH---DSHMTMALGTL----MLLKKQPE-LPKGTIRFIFQPAEEKGG--GALKMIEEGVLDDIDYLYGVHVRPIQETQNGRCAPSILHGSSQHIEGTIIGEEAHGARPHLGKNSIEIAAFLVHKLGLIHI---DPQIPHTVKMTKLQAGGESSNIIPGKASFSLDLRAQTNEAMEALIAETERACEAAAAAFGAKIELHKEHSLPA-ATQNKEAEAIMAEAITEIIGAERLDDPLVTTGGEDFHFYAV-KVPNLKTTMLGLGCGLQPGLHHPHMTFDRNAMFTGIHILANAVLKTFQKAESLAAANAS 2RB7 Chain:A ((3-360)) ------SSMQHIVELTSDLIRFPSMHSRPEQISRCAGFIMDWCAQNGIHAERMDHDGIPSVMVLPEKGRAGLLLMAHIDVVDAEDDLFVPRVENDRLYGRGANDDKYAVALGLVMFRDRLNALKAAGRSQKDMALGLLITGDEEIGGMNGAAKALPL---IRADYVVALDGGN----P-QQ--VITKEKGIIDIKLTCTGKAAHGARPWMGVNAVDLLMEDYTRLKTLFAEENEDHWHRTVNLGRIRAGE-STNKVPDVAEGWFNIRVTEHDDPGALIDKIRKTVSGT-------VSIV--RTVPVFLAADSPYTERLLALSGA----T----AGKAHGASDARYLGENGLTG---VVWGAEGF--NTLHSRDECLHIPSLQSIYDPLMQLAREMEE-----------

General information: TITO was launched using: RESULT: Template: 2RB7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1825 -165169 -90.50 -496.00 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -90.50 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_2RB7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RB7-query.scw PDB file : Tito_Scwrl_2RB7.pdb: