Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELFEAYKKGKFF-FITKFG-QQPHTSF-DYTDLDEDYFFVFGRETSGLPKDLI-QNNMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK 4PZK Chain:A ((2-156)) ----GVHVVLYQPEIPANTGNIARTCAATGTELHLIRPLGFSTDDKMLKRAGLDYWQHVKITYYDSIEEFYEKNKDGEFFYLTKYGEKAHTAFDYSKRE---KDYYFVFGRETNGLPANVIEE-NFDHCLRIPMTDKVRSLNLSNTAAILIYEAFRQQNYPGL-

General information: TITO was launched using: RESULT: Template: 4PZK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 687 -112447 -163.68 -744.68 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -163.68 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_4PZK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PZK-query.scw PDB file : Tito_Scwrl_4PZK.pdb: