Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKFFEYNWQVRDQWFTWCHQLTTEELLKNRLGGVENILYTLFHIIDVEYSWIRAI--QGKEDI-A------------VQ--F--ADYQTLNKVKSLSNTFRTEIIDVLQTHSDQIKDELVSVPWETGVLYTRDEILHHIIAHEIHHIGQLSVWARELKLSPVSASFIGRTLKPIHSY 4X8E Chain:A ((11-154)) -AELARQLIDARNRTLRLV-DFDDAELRRQYDPLMSPLVWDLAHIGQQEELWLLRGGDPRRPGLLEPAVEQLYDAFVHPRASRVHLPLLSPAQARRFCATVRSAVLDALDRLP----------------EDADTFAFGMVVSHEHQHDETMLQALNLRSGEP----------------

General information: TITO was launched using: RESULT: Template: 4X8E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 442 -58838 -133.12 -470.70 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -133.12 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4X8E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X8E-query.scw PDB file : Tito_Scwrl_4X8E.pdb: