Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTEDHSYKDKKVISIGIVSELTGLSVRQIRYYEER-KLIYPQRSSRGTRKYSFADVERLMDIANKREDGVQTAEILKDMRKKEQMLKNDPQVRKKMLEGQLNAHFRYKNR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 5C8D Chain:F ((25-296)) -----------GVYTIAEVEAMTGLSAEVLRQWERRYGFPKPRRTPH--RLYSAEDVEALKTIKRWLEEGATPKAAIRRYLAQEV--------RPEDLGTGLLEALLRGDLAGAEALFRRGLRFWGPEGVLEHLLLPVLREVGEAWHRGEIGVAEEHLASTFLRARLQELLDLAGFPPGPPVLVTTPPGERHEIGAMLAAYHLRRKGVPALYLGPDTPLPDLRALARRLGAGAVVLSAVLSEPLRALPDGALKDLAPRVFLGGQGAGPEEARRLGAEYMEDLKGLAEALWL

General information: TITO was launched using: RESULT: Template: 5C8D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 262 -21234 -81.04 -238.58 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain F : 0.55 3D Compatibility (PKB) : -81.04 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.690

(partial model without unconserved sides chains): PDB file : Tito_5C8D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C8D-query.scw PDB file : Tito_Scwrl_5C8D.pdb: